Human Survivin Quantikine ELISA Kit

  (9 citations)     
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Assay Procedure
Citations (9)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
  • Sensitivity
    9.96 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human Survivin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23 , Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human Survivin immunoassay is a 4.5 hour solid phase ELISA designed to measure human Survivin in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human Survivin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Survivin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Survivin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 137 398 841 164 445 879
Standard Deviation 6.41 27.3 44.2 14.6 39.4 63.8
CV% 4.7 6.9 5.3 8.9 8.9 7.3

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 190 513 982 197 529 1060
Standard Deviation 7.78 17.4 53.8 18.8 37.3 59.9
CV% 4.1 3.4 5.5 9.5 7.1 5.6

Recovery

The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-109
EDTA Plasma (n=4) 96 89-109
Heparin Plasma (n=4) 96 87-110
Serum (n=4) 93 85-109
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Survivin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Survivin [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.

  • Entrez Gene IDs:
    332 (Human)
  • Alternate Names:
    API4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4; Survivin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Sample Type
  1. Racial differences in the expression of inhibitors of apoptosis (IAP) proteins in extracellular vesicles (EV) from prostate cancer patients
    Authors: S Khan, J Simpson, JC Lynch, D Turay, S Mirshahidi, A Gonda, TW Sanchez, CA Casiano, NR Wall
    PLoS ONE, 2017;12(10):e0183122.
    Species: Human
    Sample Type: Plasma
  2. Evaluation of tissue metalloproteinase inhibitor TIMP-1 and Survivin levels during third trimester pregnancy - a preliminary report
    Authors: A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu
    Ginekol. Pol., 2017;88(4):198-204.
    Species: Human
    Sample Type: Serum
  3. Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients
    Oncol Lett, 2016;12(5):3591-3597.
    Species: Human
    Sample Type: Serum
  4. Serum survivin increases in prolactinoma.
    Authors: Dellal, Fatma Di, Niyazoglu, Mutlu, Gorar, Suheyla, Ademoglu, Esranur, Candan, Zehra, Bekdemir, Handan, Hacioglu, Yalcin, Kaya, Fatih On
    J Clin Med Res, 2015;7(4):248-52.
    Species: Human
    Sample Type: Serum
  5. Prognostic significance of pretreatment VEGF, survivin, and Smac/DIABLO serum levels in patients with serous ovarian carcinoma.
    Authors: Dobrzycka B, Mackowiak-Matejczyk B, Terlikowska K, Kulesza-Bronczyk B, Kinalski M, Terlikowski S
    Tumour Biol, 2015;0(0):4157.
    Species: Human
    Sample Type: Serum
  6. Early diagnostic value of survivin and its alternative splice variants in breast cancer.
    Authors: Khan S, Bennit H, Turay D, Perez M, Mirshahidi S, Yuan Y, Wall N
    BMC Cancer, 2015;14(0):176.
    Species: Human
    Sample Type: Serum
  7. Clinical significance of serum survivin in patients with pancreatic ductal adenocarcinoma.
    Authors: Ren, Y-Q, Zhang, H-Y, Su, T, Wang, X-H, Zhang, L
    Eur Rev Med Pharmacol Sci, 2014;18(20):3063-8.
    Species: Human
    Sample Type: Serum
  8. Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.
    Authors: Khan, Salma, Jutzy, Jessica, Valenzuela, Malyn Ma, Turay, David, Aspe, Jonathan, Ashok, Arjun, Mirshahidi, Saied, Mercola, Dan, Lilly, Michael, Wall, Nathan R
    PLoS ONE, 2012;7(10):e46737.
    Species: Human
    Sample Type: Plasma
  9. Extracellular, cell-permeable survivin inhibits apoptosis while promoting proliferative and metastatic potential.
    Authors: Khan S, Aspe JR, Asumen MG, Almaguel F, Odumosu O, Acevedo-Martinez S, De Leon M, Langridge WH, Wall NR
    Br. J. Cancer, 2009;100(7):1073-86.
    Species: Human
    Sample Type: Cell Culture Supernates

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