Human Survivin Quantikine ELISA Kit

R&D Systems | Catalog # DSV00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)

Sensitivity

9.96 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human Survivin Quantikine ELISA Kit

The Quantikine Human Survivin immunoassay is a 4.5 hour solid phase ELISA designed to measure human Survivin in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human Survivin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Survivin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Survivin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Survivin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 137 398 841 164 445 879
Standard Deviation 6.41 27.3 44.2 14.6 39.4 63.8
CV% 4.7 6.9 5.3 8.9 8.9 7.3

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 190 513 982 197 529 1060
Standard Deviation 7.78 17.4 53.8 18.8 37.3 59.9
CV% 4.1 3.4 5.5 9.5 7.1 5.6

Recovery for Human Survivin Quantikine ELISA Kit

The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-109
EDTA Plasma (n=4) 96 89-109
Heparin Plasma (n=4) 96 87-110
Serum (n=4) 93 85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Survivin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Survivin ELISA Linearity

Scientific Data Images for Human Survivin Quantikine ELISA Kit

Human Survivin ELISA Cell Culture Supernate/Urine Standard Curve

Human Survivin ELISA Cell Culture Supernate/Urine Standard Curve

Human Survivin ELISA Serum/Plasma Standard Curve

Human Survivin ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human)

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Human Survivin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Survivin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Survivin Quantikine ELISA Kit

Customer Reviews for Human Survivin Quantikine ELISA Kit (4)

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Survivin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 03/28/2022
    Human Survivin Quantikine ELISA Kit DSV00
  • Human Survivin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 01/03/2022
    R^2 is great, but the absorbance values are not. Most of the samples were below the zero.
    Human Survivin Quantikine ELISA Kit DSV00
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human Survivin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma and Cell Culture Media
    Verified Customer | Posted 07/27/2021
    Human Survivin Quantikine ELISA Kit DSV00
  • Human Survivin Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 08/17/2018
    Human Survivin Quantikine ELISA Kit DSV00

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Protocols

View specific protocols for Human Survivin Quantikine ELISA Kit (DSV00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail