Human Survivin Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
Control Products Available
Human Survivin ELISA Cell Culture Supernate/Urine Standard Curve
3 Images
Product Details
Citations (10)
Supplemental Products
Reviews (4)

Human Survivin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
9.96 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Natural and recombinant human Survivin
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Survivin immunoassay is a 4.5 hour solid phase ELISA designed to measure human Survivin in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human Survivin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Survivin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Survivin.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 137 398 841 164 445 879
Standard Deviation 6.41 27.3 44.2 14.6 39.4 63.8
CV% 4.7 6.9 5.3 8.9 8.9 7.3

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 190 513 982 197 529 1060
Standard Deviation 7.78 17.4 53.8 18.8 37.3 59.9
CV% 4.1 3.4 5.5 9.5 7.1 5.6


The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-109
EDTA Plasma (n=4) 96 89-109
Heparin Plasma (n=4) 96 87-110
Serum (n=4) 93 85-109


To assess the linearity of the assay, samples spiked with high concentrations of Survivin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Survivin ELISA Linearity

Scientific Data

Human Survivin ELISA Cell Culture Supernate/Urine Standard Curve

Human Survivin ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.

Entrez Gene IDs:
332 (Human)
Alternate Names:
API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Survivin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Survivin is a negative prognostic factor in malignant pleural effusion
    Authors: E Arellano-O, B Romero-Rom, V Sánchez-Ló, J Martín-Jua, F Rodríguez-, R Otero-Cand
    Eur. J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Pleural Fluid
  2. Racial differences in the expression of inhibitors of apoptosis (IAP) proteins in extracellular vesicles (EV) from prostate cancer patients
    Authors: S Khan, J Simpson, JC Lynch, D Turay, S Mirshahidi, A Gonda, TW Sanchez, CA Casiano, NR Wall
    PLoS ONE, 2017;12(10):e0183122.
    Species: Human
    Sample Types: Plasma
  3. Evaluation of tissue metalloproteinase inhibitor TIMP-1 and Survivin levels during third trimester pregnancy - a preliminary report
    Authors: A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu
    Ginekol. Pol., 2017;88(4):198-204.
    Species: Human
    Sample Types: Serum
  4. Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients
    Oncol Lett, 2016;12(5):3591-3597.
    Species: Human
    Sample Types: Serum
  5. Prognostic significance of pretreatment VEGF, survivin, and Smac/DIABLO serum levels in patients with serous ovarian carcinoma.
    Authors: Dobrzycka B, Mackowiak-Matejczyk B, Terlikowska K, Kulesza-Bronczyk B, Kinalski M, Terlikowski S
    Tumour Biol, 2015;0(0):4157.
    Species: Human
    Sample Types: Serum
  6. Serum survivin increases in prolactinoma.
    Authors: Dellal, Fatma Di, Niyazoglu, Mutlu, Gorar, Suheyla, Ademoglu, Esranur, Candan, Zehra, Bekdemir, Handan, Hacioglu, Yalcin, Kaya, Fatih On
    J Clin Med Res, 2015;7(4):248-52.
    Species: Human
    Sample Types: Serum
  7. Early diagnostic value of survivin and its alternative splice variants in breast cancer.
    Authors: Khan S, Bennit H, Turay D, Perez M, Mirshahidi S, Yuan Y, Wall N
    BMC Cancer, 2015;14(0):176.
    Species: Human
    Sample Types: Serum
  8. Clinical significance of serum survivin in patients with pancreatic ductal adenocarcinoma.
    Authors: Ren, Y-Q, Zhang, H-Y, Su, T, Wang, X-H, Zhang, L
    Eur Rev Med Pharmacol Sci, 2014;18(20):3063-8.
    Species: Human
    Sample Types: Serum
  9. Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.
    Authors: Khan, Salma, Jutzy, Jessica, Valenzuela, Malyn Ma, Turay, David, Aspe, Jonathan, Ashok, Arjun, Mirshahidi, Saied, Mercola, Dan, Lilly, Michael, Wall, Nathan R
    PLoS ONE, 2012;7(10):e46737.
    Species: Human
    Sample Types: Plasma
  10. Extracellular, cell-permeable survivin inhibits apoptosis while promoting proliferative and metastatic potential.
    Authors: Khan S, Aspe JR, Asumen MG, Almaguel F, Odumosu O, Acevedo-Martinez S, De Leon M, Langridge WH, Wall NR
    Br. J. Cancer, 2009;100(7):1073-86.
    Species: Human
    Sample Types: Cell Culture Supernates


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Reviews for Human Survivin Quantikine ELISA Kit

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Human Survivin Quantikine ELISA Kit
By Anonymous on 03/28/2022
Sample Tested: Cell culture supernatant

Human Survivin Quantikine ELISA Kit
By Anonymous on 01/03/2022
Sample Tested: Serum

R^2 is great, but the absorbance values are not. Most of the samples were below the zero.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Human Survivin Quantikine ELISA Kit
By Anonymous on 07/27/2021
Sample Tested: Cell Culture Media,EDTA Plasma

Human Survivin Quantikine ELISA Kit
By Adam Guess on 08/17/2018
Sample Tested: Cell Culture Supernates