Human Syndecan-2/CD362 PE-conjugated Antibody

Clone 305515 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2965P-100
FAB2965P-025
Detection  of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry.
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Human Syndecan-2/CD362 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Syndecan-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombiant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.
Source
Monoclonal Rat IgG2B Clone # 305515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-2
Glu19-Gly144
Accession # AAH49836.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 PE‑conjugated Monoclonal Antibody (Catalog # FAB2965P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGFbasic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

References
  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.
Entrez Gene IDs
6383 (Human); 15529 (Mouse)
Alternate Names
CD362 antigen; CD362; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; HSPG1; HSPG1syndecan proteoglycan 2; SDC2; SYND2; SYND2cell surface-associated heparan sulfate proteoglycan 1; syndecan 2; Syndecan2; Syndecan-2

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