Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

R&D Systems | Catalog # BAM9303

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 224964
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Arg215-Asn671 (predicted)
Accession # P78536

Specificity

Detects the ectodomain of human TACE/ADAM17 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse (rm) TACE, recombinant human (rh) ADAM8, rhADAM9, or rmADAM10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

Human TACE / ADAM17 ELISA Standard Curve

Human TACE / ADAM17 ELISA Standard Curve

Recombinant Human TACE/ADAM17 (Catalog # 930-ADB) was serially diluted and captured by Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9304) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TACE/ADAM17 Ectodomain Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

Human TACE/ADAM17 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TACE/ADAM17 Ectodomain Antibody (Catalog # MAB9304)
  • Standard: Recombinant Human TACE/ADAM17 Protein, CF (Catalog # 930-ADB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References

  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

For research use only

Customer Reviews for Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]

There are currently no reviews for this product. Be the first to review Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin] and earn rewards!

Have you used Human TACE/ADAM17 Ectodomain Antibody (224964) [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies