TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.
Recombinant Human TACE/ADAM17 Protein, CF
R&D Systems | Catalog # 930-ADB
Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Human TACE/ADAM17 Protein (930-ADB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Arg215-Asn671, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 4 using 930-ADB in the following applications:
Scientific Data Images for Recombinant Human TACE/ADAM17 Protein, CF
Recombinant Human TACE/ADAM17 Protein SEC-MALS.
Recombinant Human TACE/ADAM17 Protein (Catalog # 930-ADB) has a molecular weight (MW) of 58-64 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Formulation, Preparation, and Storage
930-ADB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Brij-35. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile, deionized water.
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| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: TACE/ADAM17
References
- Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
- Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TACE/ADAM17 Products
Product Documents for Recombinant Human TACE/ADAM17 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human TACE/ADAM17 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human TACE/ADAM17 Protein, CF
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Application: Enzymatic activity in vitroVerified Customer | Posted 10/09/2019
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Protocols
View specific protocols for Recombinant Human TACE/ADAM17 Protein, CF (930-ADB):
- Assay Buffer: 25 mM Tris, 2.5 μM ZnCl2, 0.005% Brij-35 (w/v), pH 9.0 (note: It is extremely important that the assay solution does not contain salt (CaCl2, NaCl, Na2SO4) because it inhibits TACE activity).
- Recombinant Human TACE/ADAM17 (rhTACE) (Catalog # 930-ADB)
- Substrate: MCA-Pro-Leu-Ala-Gln-Ala-Val-DPA-Arg-Ser-Ser-Ser-Arg-NH2 (Catalog # ES003), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhTACE to 0.2 ng/µL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- In a plate load 50 µL of 0.2 ng/µL rhTACE and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 µL Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
FAQs for Recombinant Human TACE/ADAM17 Protein, CF
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Q: If the Recombinant Human TACE/ADAM17 Protein, CF (Catalog # 930-ADB) is supplied in solution consisting of 25 mM Tris, 200 mM NaCl and 0.02%(w/v) Brij-35, does the 200 mM NaCl in the formulation inhibit the activity of the protein?
A: We would not expect the 200 mM NaCl salt to affect the stability of the 930-ADB enzyme. The reconstituted material will be with DI water and is generally further diluted >1000-fold for assays, so the inhibition by NaCl becomes negligible and no impact in the activity has been observed. Ample dilution of the enzyme is necessary to ensure there are no negative effects from the salt in the formulation.
Associated Pathways
Notch Signaling Pathways
