Human TAF5L Alexa Fluor® 532-conjugated Antibody

Catalog # Availability Size / Price Qty
AF6785X-100UG

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Human TAF5L Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TAF5L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TAF1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TAF5L
Met1-Tyr189
Accession # O75529
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TAF5L

TAF5L (TBP-Associated Factors 5-Like; also RNA polymerase II p300-associated factor 65 kDa subunit 5L, PACF65 beta, PAF65 beta and STAF65 beta ) is a 64-66 kDa member of the TAF5 family of proteins. It is a nuclear protein that forms part of the PCAF, STAGA and TFTC histone acetylase complexes. The acetylases within these complexes acetylate hisone H3 within nucleosomes, allowing for the recognition and binding of transcriptional activators. Human TAF5L is 589 amino acids (aa) in length. It contains an N-terminal homodimerization interface (aa 90-198) plus six consecutive WD repeats (aa 266-547). There is one potential splice form that shows a Val substitution for aa 325-589. Over aa 1-189, human TAF5L shares 94% aa identity with mouse TAF5L.

Long Name
TAF5-like RNA Polymerase II p300/CBP-associated Factor-associated factor 65 kDa Subunit 5L
Entrez Gene IDs
27097 (Human); 102162 (Mouse); 307927 (Rat)
Alternate Names
PAF65B; PAF65BPAF65-beta; PCAF associated factor 65 beta; PCAF-associated factor 65 beta; TAF5L; TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDasubunit 5L; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65 kD; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65kDa

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