Detection of Human TAF5L by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line, 293T human embryonic kidney cell line, and U2OS human osteosarcoma cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TAF5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TAF5L at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TAF5L (TBP-Associated Factors 5-Like; also RNA polymerase II p300-associated factor 65 kDa subunit 5L, PACF65 beta, PAF65 beta and STAF65 beta ) is a 64-66 kDa member of the TAF5 family of proteins. It is a nuclear protein that forms part of the PCAF, STAGA and TFTC histone acetylase complexes. The acetylases within these complexes acetylate hisone H3 within nucleosomes, allowing for the recognition and binding of transcriptional activators. Human TAF5L is 589 amino acids (aa) in length. It contains an N-terminal homodimerization interface (aa 90-198) plus six consecutive WD repeats (aa 266-547). There is one potential splice form that shows a Val substitution for aa 325-589. Over aa 1-189, human TAF5L shares 94% aa identity with mouse TAF5L.
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