Human TEM5/GPR124 Antibody

Human TEM5/GPR124 MAb (Clone 499708)
Catalog # Availability Size / Price Qty
Detection of TEM5/GPR124 in CHO-S Transfected (positive) and CHO-S Wild Type (negative) Cells.
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Product Details

Human TEM5/GPR124 Antibody Summary

Species Reactivity
Detects human TEM5/GPR124 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 499708
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124
Accession # Q96PE1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Scientific Data

Immunocytochemistry View Larger

Detection of TEM5/GPR124 in CHO-S Transfected (positive) and CHO-S Wild Type (negative) Cells. TEM5/GPR124 was detected in immersion fixed CHO-S transfected (positive) and CHO-S wild type (negative) cells using Mouse Anti-Human TEM5/GPR124 Monoclonal Antibody (Catalog # MAB88961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM5/GPR124

G protein-coupled receptor 124 (GPR124, also known as TEM5), is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. GPR124 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. This receptor mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif.

Long Name
Tumor Endothelial Marker 5
Entrez Gene IDs
25960 (Human); 78560 (Mouse)
Alternate Names
DKFZp434C211; DKFZp434J0911; G protein-coupled receptor 124; GPR124; G-protein coupled receptor 124; KIAA1531; KIAA1531FLJ14390; TEM5; TEM5Tumor endothelial marker 5

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