Human TfR (Transferrin R) Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2474T-100UG
Detection of TfR (Transferrin R) in U937 Human Cell Line by Flow Cytometry.
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Human TfR (Transferrin R) Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TfR in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 29806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human TfR
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TfR (Transferrin R) in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of TfR (Transferrin R) in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human TfR (Transferrin R) Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB2474T, filled histogram) or isotype control antibody (Catalog # IC002T, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TfR (Transferrin R)

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1‑4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Human TfR ECD shares 75‑80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region (6). Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 7).

References
  1. Schneider, C. et al. (1984) Nature 311:675.
  2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
  3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
  4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
  5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
  6. Entrez protein Accession # EAW53671, EAW53672.
  7. Radoshitzky, S.R. et al. (2007) Nature 446:92.
Long Name
Transferrin Receptor
Entrez Gene IDs
7037 (Human); 22042 (Mouse); 64678 (Rat)
Alternate Names
CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR

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