Human TfR (Transferrin R) PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TfR in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 29806
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human TfR
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TfR (Transferrin R) in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human TfR (Transferrin R) PE‑conjugated Monoclonal Antibody (Catalog # FAB2474P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TfR (Transferrin R)

The Transferrin Receptor (TfR) also known as TfR-1 and CD71 is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1-4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Human TfR ECD shares 75-80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region (6). Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and Galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 7).

  • References:
    1. Schneider, C. et al. (1984) Nature 311:675.
    2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
    3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
    4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
    5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
    6. Entrez protein Accession # EAW53671, EAW53672.
    7. Radoshitzky, S.R. et al. (2007) Nature 446:92.
  • Long Name:
    Transferrin Receptor
  • Entrez Gene IDs:
    7037 (Human); 22042 (Mouse); 64678 (Rat)
  • Alternate Names:
    CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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