Home / TGF-beta RII / Human TGF-beta  RII Fluorescein-conjugated Antibody

Human TGF-beta  RII Fluorescein-conjugated Antibody

Catalog #: FAB241F
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB241F-100
FAB241F-025
Detection of TGF-beta RII in NS0 Mouse Cell Line Transfected with TGF-beta RII by Flow Cytometry.
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Citations (4)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta  RII Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
This antibody has been validated by flow cytometry on TGF-beta RII-transfected cells only. In contrast to Catalog # FAB2411F, minimal staining of PBMC lymphocytes was observed with this antibody.
Source
Monoclonal Mouse IgG1 Clone # 25508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
Ile24-Asp159
Accession # P37173.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TGF-beta RII antibody in NS0 Mouse Cell Line Transfected with TGF-beta RII antibody by Flow Cytometry. View Larger

Detection of TGF-beta RII in NS0 Mouse Cell Line Transfected with TGF-beta RII by Flow Cytometry. NS0 mouse myeloma cell line transfected with TGF-beta RII was stained with Mouse Anti-Human TGF-beta RII Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB241F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RII

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

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Citations for Human TGF-beta  RII Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Oral fibroblasts modulate the macrophage response to bacterial challenge
    Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
    Sci Rep, 2017;7(1):11516.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  2. Extracellular Hsp90 and TGF? regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model
    Authors: JA de la Mare, T Jurgens, AL Edkins
    BMC Cancer, 2017;17(1):202.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination
    Authors: D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells.
    Authors: Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z
    Stem Cells, 2005;24(1):65-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Mouse IgG1 Fluorescein-conjugated Antibody

IC002F
24 Citations
2 Reviews
1 Image
Ctrl

Staining Reagents

Flow Cytometry Human Lyse Buffer (10X)

FC002
2 Citations
1 Review
Flow

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
26 Citations
5 Reviews
Flow

Reviews for Human TGF-beta  RII Fluorescein-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

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Human TGF-beta  RII Fluorescein-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: NK-92 human natural killer lymphoma cell line Species: Human

Intra-cellular staining

Flow Cytometry Human TGF-beta  RII Fluorescein-conjugated Antibody FAB241F