Human Thrombomodulin/BDCA-3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Thrombomodulin/BDCA-3 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 501733
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Thrombomodulin/BDCA-3
    Accession # P07204(Val473)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection ofThrombomodulin/BDCA‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-HumanThrombomodulin/BDCA‑3 APC‑conjugated Monoclonal Antibody(Catalog # FAB3947A) and Mouse Anti-Human HLA‑DRFluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4869F).Quadrant markers were set based on isotype control antibody staining. View ourprotocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as Blood Dendritic Cell Antigen 3 (BDCA‑3) and designated CD141. The deduced amino acid (aa) sequence of human THBD predicts a signal peptide (aa 1‑18) and a mature chain (aa 19‑575) that consists of the following domains: a C‑type lectin domain (aa 31‑169), EGF‑like domains (aa 241‑281, aa 284‑324, aa 325‑363, aa 365‑405, aa 404‑440, and aa 441‑481), a transmembrane domain (aa 516 to 539), and a cytoplasmic region (aa 540‑575). The region used as an immunogen consists of aa 19‑515, corresponding to the extracellular portion of the type I transmembrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1‑3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which, in turn, activates Protein C and Thrombin‑activatable Fibrinolysis Inhibitor (TAFI), also known as Carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by Protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C‑terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisms are associated with human disease and with THBD playing a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD due to protease cleavage have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).

  • References:
    1. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:1374.
    2. Wu, K.K. et al. (2000) Ann. Med. 32:73.
    3. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
    4. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
    5. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
  • Entrez Gene IDs:
    7056 (Human); 21824 (Mouse); 83580 (Rat)
  • Alternate Names:
    BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; thrombomodulin; TM
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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