hh Human Thrombomodulin/BDCA-3 Antibody MAB3947-100: R&D Systems

Human Thrombomodulin/BDCA-3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Thrombomodulin/BDCA-3 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 501733
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Thrombomodulin/BDCA-3
    Ala19-Ser515
    Accession # P07204(Val473)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human Thrombomodulin/BDCA-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Thrombomodulin/BDCA‑3 Antibody (Catalog # MAB3947)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human Thrombomodulin/BDCA‑3 Biotinylated Antibody (Catalog # BAM39471)
  • ELISA Standard
     
    Recombinant Human Thrombomodulin/BDCA-3 Protein, CF (Catalog # 3947-PA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Thrombomodulin/BDCA‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human HLA‑DR APC‑conjugated Monoclonal Antibody (Catalog # FAB4869A) and either (A) Mouse Anti-Human Thrombomodulin/BDCA‑3 Monoclonal Antibody (Catalog # MAB3947) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
Thrombomodulin/BDCA-3 in Human Kidney. Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as blood dendritic cell antigen 3 (BDCA‑3) and designated CD141. The deduced amino acid (aa) sequence of human THBD predicts a signal peptide (aa 1‑18) and a mature chain (aa 19‑575) that consists of following domains: C‑type lectin (aa 31‑169), EGF‑like (aa 241‑281, aa 284‑324, aa 325‑363, aa 365‑405, aa 404‑440, and aa 441‑481), transmembrane (aa 516 to 539) and cytoplasmic (aa 540‑575). The region used as an immunogen consists of aa 19‑515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1‑3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin‑activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C‑terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).

  • References:
    1. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
    2. Wu, K.K. et al. (2000) Ann. Med. 32:73.
    3. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
    4. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
    5. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
  • Entrez Gene IDs:
    7056 (Human); 21824 (Mouse); 83580 (Rat)
  • Alternate Names:
    BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM
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