Human Thrombomodulin/BDCA-3 Antibody Summary
Accession # P07204(Val473)
Human Thrombomodulin/BDCA-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Thrombomodulin/BDCA‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human HLA-DR APC-conjugated Monoclonal Antibody (Catalog # FAB4869A) and either (A) Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Thrombomodulin/BDCA-3 in Human Kidney. Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Encoded by the THBD gene, Thrombomodulin is also known as blood dendritic cell antigen 3 (BDCA‑3) and designated CD141. The deduced amino acid (aa) sequence of human THBD predicts a signal peptide (aa 1‑18) and a mature chain (aa 19‑575) that consists of following domains: C‑type lectin (aa 31‑169), EGF‑like (aa 241‑281, aa 284‑324, aa 325‑363, aa 365‑405, aa 404‑440, and aa 441‑481), transmembrane (aa 516 to 539) and cytoplasmic (aa 540‑575). The region used as an immunogen consists of aa 19‑515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1‑3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin‑activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C‑terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).
- Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
- Wu, K.K. et al. (2000) Ann. Med. 32:73.
- Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
- Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
- Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
Citations for Human Thrombomodulin/BDCA-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Thrombin cleaves and activates the protease-activated receptor 2 dependent on thrombomodulin co-receptor availability
Authors: DM Heuberger, AG Franchini, J Madon, RA Schuepbach
Thromb. Res., 2019;177(0):91-101.
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture)
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
PLoS ONE, 2018;13(10):e0204177.
Sample Types: Whole Cells
Applications: Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Thrombomodulin/BDCA-3 Antibody
There are currently no reviews for this product. Be the first to review Human Thrombomodulin/BDCA-3 Antibody and earn rewards!
Have you used Human Thrombomodulin/BDCA-3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image