Human Tie-1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Tie‑2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Tie-1
    Accession # P35590
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Tie‑1 in Human Umbilical Vein Endothelial Cells (HUVEC) by Flow Cytometry. Human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB619P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Tie-1

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains, flanking three epidermal growth factor (EGF)-like domains followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain, a 25 residue transmembrane domain, and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

  • References:
    1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159
    2. Sato, T.N. et al. (1995) Nature 376:70
  • Long Name:
    Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
  • Entrez Gene IDs:
    7075 (Human); 21846 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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