Human Tie-1 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Tie-1 is observed and less than 1% cross-reactivity with recombinant human Tie‑2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Tie-1
    Ala22-Gln760
    Accession # P35590
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Tie‑1 Fc Chimera (Catalog # 619-TI)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Tie‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunohistochemistry
Tie‑1 in Human Placenta. Tie‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-1

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

  • References:
    1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159
    2. Sato, T.N. et al. (1995) Nature 376:70
  • Long Name:
    Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
  • Entrez Gene IDs:
    7075 (Human); 21846 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
    Authors: VM Leppänen, P Saharinen, K Alitalo
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015;6(0):5962.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  3. Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2.
    Authors: Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP
    J. Biol. Chem., 2007;282(42):30509-17.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells.
    Authors: Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK
    Cardiovasc. Res., 2006;72(3):394-402.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003;112(1):126-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2.
    Authors: Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP
    J. Biol. Chem., 2000;275(50):39741-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
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