Human TIM-3 Biotinylated Antibody

BAF2365 1 Citation Datasheet / COA / SDS

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Human TIM-3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIM-3 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TIM‑1, recombinant mouse (rm) TIM‑1, rmTIM‑2, and rmTIM‑3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TIM‑3 Fc Chimera (Catalog # 2365-TM)

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4⁺ Th1 and CD8⁺ Tc1) but not on helper T cells (CD4⁺ Th2 and CD8⁺ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

References

Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B. 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Monney, L. et al. (2002) Nature 415:536.
Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
Khademi, M. et al. (2004) J. Immunol. 172:7169.
Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
Oikawa, T. et al. (2006) J. Immunol. 177:4281.
Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Geng, H. et al. (2006) J. Immunol. 176:1411.

Long Name

T Cell Immunoglobulin Mucin-3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)

Alternate Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3

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Citation for Human TIM-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1