Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human TLE1
His11-Asn200
Accession # Q04724

Specificity

Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human TLE1 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TLE1

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln‑rich region that mediates oligomerization (aa 1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa 200-268), and six WD repeats that mediate protein‑protein interaction (aa 470-767). Phosphorylation on Ser will generate 90-93 kDa and 116 kDa forms in SDS‑Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10 aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa 11-200, human TLE1 shares 98% aa identity with mouse TLE1.

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Entrez Gene IDs

7088 (Human); 21885 (Mouse); 362533 (Rat)

Gene Symbol

TLE1

UniProt

Additional TLE1 Products

Product Documents for Human TLE1 Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TLE1 Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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