Human Total PON3 DuoSet IC ELISA

Name: Human Total PON3 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC4345-2

R&D Systems | Catalog # DYC4345-2

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Product Documents

Discontinued Product

DYC4345-2 has been discontinued. View all PON3 products.

Product Summary
Product Specifications
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

234-15000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human

Human Total PON3 DuoSet IC ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

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Product Summary for Human Total PON3 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human Total PON3 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PON3

PON (paraoxonase/lactonase) family members PON1, PON2 and PON3 are calcium-dependent antioxidant enzymes. PON1 is secreted into the bloodstream associated with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. The antioxidant activity of PON1 and other PON members may slow the initiation and progression of atherosclerosis.

Long Name

Paraoxonase 3

Alternate Names

EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3

Entrez Gene IDs

5446 (Human); 269823 (Mouse); 312086 (Rat)

Gene Symbol

PON3

Additional PON3 Products

Product Documents for Human Total PON3 DuoSet IC ELISA

Download Product Datasheets

Download SDS

Product Specific Notices for Human Total PON3 DuoSet IC ELISA

For research use only

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