Human TRA-1-60(R) PE-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRA‑1‑60(R).
  • Source
    Monoclonal Mouse IgM Clone # TRA-1-60
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    2102Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA‑1‑60(R) PE‑conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

  • References:
    1. Andrews, P. et al. (1984) Hybridoma 3:347.
    2. Thomson, J. et al. (1998) Science 282:1145.
    3. Giwercman, A. et al. (1993) Cancer 72:1308.
    4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
    5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
    6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
  • Long Name:
    Tumor-related Antigen-1-60 [R]
  • Entrez Gene IDs:
    5420 (Human)
  • Alternate Names:
    TRA160(R); TRA-1-60(R)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM PE-conjugated Control

Ctrl IC015P 1
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human TRA-1-60(R) PE-conjugated Antibody FAB4770P
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Excellent
 Flow Human Anonymous 12/07/2016
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Flow Cytometry Human TRA-1-60(R) PE-conjugated Antibody FAB4770P
Flow Cytometry: Human TRA-1-60(R) PE-conjugated Antibody [FAB4770P]

Summary

ApplicationFlow Cytometry
Sample TestedBreast cancer cells
SpeciesHuman

Other Experimental Details

Other Experimental Details10^6 MDA-MB-231 cells were incubated for 30 minutes at 4C with 10 ul of Mouse TRA-1-60 PE-conjugated antibody (red), or control antibody (black). Blocking: 1% BSA. Unfixed cells.

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