Human TRA-1-60(R) PE-conjugated Antibody

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Human TRA-1-60(R) PE-conjugated Antibody in Flow Cytometry
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Human TRA-1-60(R) PE-conjugated Antibody Summary

Species Reactivity
Detects human TRA‑1‑60(R).
Monoclonal Mouse IgM Clone # TRA-1-60
IgM-specific Affinity-purified from hybridoma culture supernatant
2102Ep human embryonal carcinoma cell line
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry. View Larger

Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Entrez Gene IDs
5420 (Human)
Alternate Names
TRA160(R); TRA-1-60(R)

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Human TRA-1-60(R) PE-conjugated Antibody
By Anonymous on 12/07/2016
Application: Flow Sample Tested: Breast cancer cells Species: Human

10^6 MDA-MB-231 cells were incubated for 30 minutes at 4C with 10 ul of Mouse TRA-1-60 PE-conjugated antibody (red), or control antibody (black). Blocking: 1% BSA. Unfixed cells.