Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-60
View all TRA-1-60(R) Primary Antibodies »

Product Specifications

Immunogen

2102Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑60(R).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human TRA‑1‑60(R) PE‑conjugated Antibody

Detection of TRA-1-60(R) antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRA‑1‑60(R) PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BGO1V Human Embryonic Stem Cells

Reviewed Applications

Read 1 review rated 5 using FAB4770P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

References

  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.

Long Name

Tumor-related Antigen-1-60 [R]

Alternate Names

TRA160(R)

Entrez Gene IDs

5420 (Human)

Gene Symbol

PODXL

Additional TRA-1-60(R) Products

Product Documents for Human TRA‑1‑60(R) PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRA‑1‑60(R) PE‑conjugated Antibody

For research use only

Citations for Human TRA‑1‑60(R) PE‑conjugated Antibody

Customer Reviews for Human TRA‑1‑60(R) PE‑conjugated Antibody (1)

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  • Human TRA-1-60(R) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 12/07/2016
    10^6 MDA-MB-231 cells were incubated for 30 minutes at 4C with 10 ul of Mouse TRA-1-60 PE-conjugated antibody (red), or control antibody (black). Blocking: 1% BSA. Unfixed cells.
    Human TRA‑1‑60(R) PE‑conjugated Antibody FAB4770P

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