Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA‑1‑60(R) PE‑conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
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Thomson, J. et al. (1998) Science 282:1145.
Giwercman, A. et al. (1993) Cancer 72:1308.
Marrink, J. et al. (1991) Int. J. Cancer 49:368.
Badcock, G. et al. (1999) Cancer Res. 59:4715.
Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
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