Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA‑1‑60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
TRA‑1‑60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA‑1‑60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines.
TRA‑1‑60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
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Thomson, J. et al. (1998) Science 282:1145.
Giwercman, A. et al. (1993) Cancer 72:1308.
Marrink, J. et al. (1991) Int. J. Cancer 49:368.
Badcock, G. et al. (1999) Cancer Res. 59:4715.
Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
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Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
Stem Cell Res, 2017;20(0):105-114.
Sample Type: Whole Cells
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