Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 685857
View all TRANCE/TNFSF11/RANK L Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Gly64-Asp245
Accession # AAC51762

Specificity

Detects human TRANCE/TNFSF11/RANK L in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TRANCE/TNFSF11/RANK L APC‑conjugated Antibody

Detection of TRANCE/TNFSF11/RANK L antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TRANCE/TNFSF11/RANK L in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PHA for were stained with Mouse Anti-Human IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1020P) and either (A) Mouse Anti-Human TRANCE/TNFSF11/RANK L APC-conjugated Monoclonal Antibody (Catalog # FAB6264A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of TRANCE/TNFSF11/RANK L antibody in HEK293 human cell line transfected with human TRANCE antibody by Flow Cytometry.

Detection of TRANCE/TNFSF11/RANK L in HEK293 human cell line transfected with human TRANCE by Flow Cytometry.

Human HEK293 cell line transfected with human TRANCE was stained with Mouse Anti-Human TRANCE/TNFSF11/RANK L APC-conjugated Monoclonal Antibody (Catalog # FAB6264A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A, open histogram).View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRANCE/TNFSF11/RANK L APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA, NTERA2 embryonal carcinoma cell or HEK293 human cell line transfected with human TRANCE

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANK L, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family.  Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation.  Splicing variants produce three isoforms for human TRANCE.  Isoform 1 is the standard form.  In isoform 2, aa corresponding to 1-73 in isoform 1 are missing.  In isoform 3, aa 1-47 are missing.  Human TRANCE ECD is 83% identical to mouse TRANCE ECD.  TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.  TRANCE plays a role in osteoclast differentiation and activation. 

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

AAC51762

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Human TRANCE/TNFSF11/RANK L APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human TRANCE/TNFSF11/RANK L APC‑conjugated Antibody

For research use only

Citations for Human TRANCE/TNFSF11/RANK L APC‑conjugated Antibody

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