Human TRANCE/TNFSF11/RANK L APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TRANCE/TNFSF11/RANK L in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 685857
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
    Accession # AAC51762
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRANCE/TNFSF11/RANK L in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA for were stained with Mouse Anti-Human IL‑2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1020P) and either (A) Mouse Anti-Human TRANCE/TNFSF11/RANK L APC‑conjugated Monoclonal Antibody (Catalog # FAB6264A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
TNF-related activation-induced cytokine (TRANCE; also RANK L, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family.  Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation.  Splicing variants produce three isoforms for human TRANCE.  Isoform 1 is the standard form.  In isoform 2, aa corresponding to 1-73 in isoform 1 are missing.  In isoform 3, aa 1-47 are missing.  Human TRANCE ECD is 83% identical to mouse TRANCE ECD.  TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.  TRANCE plays a role in osteoclast differentiation and activation. 
  • Long Name:
    TNF-related Activation-induced Cytokine
  • Entrez Gene IDs:
    8600 (Human); 21943 (Mouse)
  • Alternate Names:
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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