Human CD25/IL-2R alpha PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1020P-100
FAB1020P-025
Human CD25/IL-2R alpha  PE-conjugated Antibody in Flow Cytometry
1 Image
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human CD25/IL-2R alpha PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
Source
Monoclonal Mouse IgG2A Clone # 24212
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Human CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. View Larger

Detection of Human CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

Citations for Human CD25/IL-2R alpha PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases
    Authors: I Peluso, A Raguzzini, G Catasta, V Cammisotto, A Perrone, C Tomino, E Toti, M Serafini
    Oxid Med Cell Longev, 2018;2018(0):5417165.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  2. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
    Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
    J Immunol, 2014;192(1):123-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
    Authors: Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M
    Eur. J. Immunol., 2010;40(1):197-203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009;133(2):257-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
    Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
    FEBS Lett., 2007;581(7):1317-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CD25/IL-2R alpha PE-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CD25/IL-2R alpha PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CD25/IL-2 R alpha PE-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Human CD25/IL-2R alpha PE-conjugated Antibody
By Anonymous on 04/26/2017
Application: