TREM‑2 in Human PBMCs. TREM‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS using Goat Anti-Human TREM‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREM-2 is a single Ig domain receptor expressed by cells of myeloid
lineage. It is a pattern recognition receptor that binds anionic
ligands. TREM-2 associates with the adapter protein DAP12 to deliver an
activating signal that plays a role in both innate and adaptive immune
Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs:
TREM2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
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