Human TREML1/TLT-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2394G-100UG

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Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry.
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Human TREML1/TLT-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.
Source
Monoclonal Rat IgG2B Clone # 268420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
Gln16-Ser160
Accession # Q86YW5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TREML1/TLT-1 antibody in Human Platelets antibody by Flow Cytometry. View Larger

Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC-conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Rat Anti-Human TREML1/TLT-1 Alexa Fluor® 488 Monoclonal Antibody (Catalog # FAB2934G) or (B) Rat IgG2B Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREML1/TLT-1

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 1
Entrez Gene IDs
340205 (Human); 71326 (Mouse)
Alternate Names
dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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