Human TREML1/TLT-1 Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 268420
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
    Gln16-Ser160
    Accession # Q86YW5
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Rat Anti-Human TREML1/TLT‑1 Alexa Fluor® 488 Monoclonal Antibody (Catalog # FAB2934G) or (B) Rat IgG2B Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREML1/TLT-1
TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells-like 1
  • Entrez Gene IDs:
    340205 (Human); 71326 (Mouse)
  • Alternate Names:
    dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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