Human TREML1/TLT-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2394G-100UG
Detection of  TREML1/TLT‑1 in Human Platelets by Flow Cytometry.
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Human TREML1/TLT-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.
Source
Monoclonal Rat IgG2B Clone # 268420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
Gln16-Ser160
Accession # Q86YW5
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of  TREML1/TLT‑1 in Human Platelets by Flow Cytometry. View Larger

Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Rat Anti-Human TREML1/TLT‑1 Alexa Fluor® 488 Monoclonal Antibody (Catalog # FAB2934G) or (B) Rat IgG2B Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREML1/TLT-1

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 1
Entrez Gene IDs
340205 (Human); 71326 (Mouse)
Alternate Names
dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1

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