Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody

FAB2394R 1 Citation Datasheet / COA / SDS

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FAB2394R-100UG

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Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry.

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Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.

Source

Monoclonal Rat IgG_2B Clone # 268420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
Gln16-Ser160
Accession # Q86YW5

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with (A) Rat Anti-Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB2394R) or (B) Rat IgG2B Isotype Control (Catalog # IC013R) and Mouse Anti-Human Integrin alpha 2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREML1/TLT-1

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Alternate Names

dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
Authors: T Vögtle, S Sharma, J Mori, Z Nagy, D Semeniak, C Scandola, MJ Geer, CW Smith, J Lane, S Pollack, R Lassila, A Jouppila, AJ Barr, DJ Ogg, TD Howard, HJ McMiken, J Warwicker, C Geh, R Rowlinson, WM Abbott, A Eckly, H Schulze, GJ Wright, A Mazharian, K Fütterer, S Rajesh, MR Douglas, YA Senis
Elife, 2019;8(0):.
Species: Mouse
Sample Types: Whole Blood
Applications: Flow Cytometry