Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody

FAB2394R 1 Citation Datasheet / COA / SDS

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FAB2394R-100UG

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Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody in Flow Cytometry

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Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.

Source

Monoclonal Rat IgG_2B Clone # 268420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
Gln16-Ser160
Accession # Q86YW5

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with (A) Rat Anti-Human TREML1/TLT‑1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB2394R) or (B) Rat IgG2B Isotype Control (Catalog # IC013R) and Mouse Anti-Human Integrin alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREML1/TLT-1

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Alternate Names

dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human TREML1/TLT-1 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
Authors: T Vögtle, S Sharma, J Mori, Z Nagy, D Semeniak, C Scandola, MJ Geer, CW Smith, J Lane, S Pollack, R Lassila, A Jouppila, AJ Barr, DJ Ogg, TD Howard, HJ McMiken, J Warwicker, C Geh, R Rowlinson, WM Abbott, A Eckly, H Schulze, GJ Wright, A Mazharian, K Fütterer, S Rajesh, MR Douglas, YA Senis
Elife, 2019;8(0):.
Species: Mouse
Sample Types: Whole Blood
Applications: Flow Cytometry