Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry.
Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Rat Anti-Human TREML1/TLT‑1 Monoclonal Antibody (Catalog # MAB2394) or (B) Rat IgG2B Isotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREML1 is an inhibitory type I transmembrane protein that has two
cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A
splice variant lacking ITIMs in its cytoplasmic domain also exists.
TREML1 is found only in platelets and megakaryocytes.
Triggering Receptor Expressed on Myeloid Cells-like 1
Entrez Gene IDs:
340205 (Human); 71326 (Mouse)
dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1
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