Human TRIF/TICAM1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6216V Datasheet / COA / SDS

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FAB6216V-100UG

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All TRIF/TICAM1 Antibodies

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Human TRIF/TICAM1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIF/TICAM1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIF (aa 29‑204), rhTRAM, rhTRIM, rhTRIM5, rhTRIM21, or rhTRIM32 is observed.

Source

Monoclonal Mouse IgG2a Clone # 567212

Purification

IgM-specific Affinity-purified

Immunogen

E. coli-derived recombinant human TRIF/TICAM1
Met474-Pro618
Accession # Q8IUC6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRIF/TICAM1

TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length. It contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 474-618, human TRIF shares 53% aa identity with mouse TRIF.

Long Name

Toll-like receptor adaptor molecule 1

Entrez Gene IDs

148022 (Human); 106759 (Mouse)

Alternate Names

MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIF; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta