Detects human TRIF/TICAM1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIF (aa 29‑204), rhTRAM, rhTRIM, rhTRIM5, rhTRIM21, or rhTRIM32 is observed.
Monoclonal Mouse IgG2A Clone # 567212
IgM-specific Affinity-purified from hybridoma culture supernatant
E. coli-derived recombinant human TRIF/TICAM1 Met474-Pro618 Accession # Q8IUC6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TRIF/TICAM1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRIF/TICAM1 Monoclonal Antibody (Catalog # MAB6216) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIF/TICAM1 at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length. It contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 474-618, human TRIF shares 53% aa identity with mouse TRIF.
Toll-like receptor adaptor molecule 1
Entrez Gene IDs:
148022 (Human); 106759 (Mouse)
MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIF; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta
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