Human TRIF/TICAM1 Antibody

R&D Systems | Catalog # AF6216

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIF/TICAM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRIF/TICAM1
Lys29-Ala204
Accession # Q8IUC6

Specificity

Detects human TRIF/TICAM1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TRIF/TICAM1 Antibody

Detection of Human TRIF/TICAM1 antibody by Western Blot.

Detection of Human TRIF/TICAM1 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
TRIF/TICAM1 antibody in Raji Human Cell Line by Immunocytochemistry (ICC).

TRIF/TICAM1 in Raji Human Cell Line.

TRIF/TICAM1 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human TRIF/TICAM1 by Simple WesternTM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TRIF/TICAM1 at approximately 122 kDa (as indicated) using 25 µg/mL of Goat Anti-Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human TRIF/TICAM1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line

Simple Western

25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

0.25 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRIF/TICAM1

TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF 3, -4, and -7, as well as NF kappa B and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length and contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 29-204, human TRIF shares 62% aa identity with mouse TRIF.

Long Name

Toll-like receptor adaptor molecule 1

Alternate Names

TICAM1

Entrez Gene IDs

148022 (Human); 106759 (Mouse)

Gene Symbol

TICAM1

UniProt

Q8IUC6 (Human)

Additional TRIF/TICAM1 Products

Product Documents for Human TRIF/TICAM1 Antibody

Certificate of Analysis

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Product Specific Notices for Human TRIF/TICAM1 Antibody

For research use only

Citations for Human TRIF/TICAM1 Antibody

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Protocols

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