Human TRIF/TICAM1 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TRIF/TICAM1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TRIF/TICAM1
    Lys29-Ala204
    Accession # Q8IUC6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TRIF/TICAM1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
TRIF/TICAM1 in Raji Human Cell Line. TRIF/TICAM1 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRIF/TICAM1

TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF 3, -4, and -7, as well as NF kappa B and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length and contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 29-204, human TRIF shares 62% aa identity with mouse TRIF.

  • Long Name:
    Toll-like receptor adaptor molecule 1
  • Entrez Gene IDs:
    148022 (Human); 106759 (Mouse)
  • Alternate Names:
    MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIF; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
    Authors: Xiang Z, Li L, Lei X, Zhou H, Zhou Z, He B, Wang J
    J Virol, 2014;88(12):6650-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
    Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
    PLoS ONE, 2014;9(5):e98517.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. TRIM38 Negatively Regulates TLR3-Mediated IFN-beta Signaling by Targeting TRIF for Degradation.
    Authors: Xue Q, Zhou Z, Lei X, Liu X, He B, Wang J, Hung T
    PLoS ONE, 2012;7(10):e46825.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
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