Detection of TSPAN33 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with overnight with Recombinant Human IL‑4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) for were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human TSPAN33 PE‑conjugated Monoclonal Antibody (Catalog # FAB8405P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TSPAN33 is a homodimeric disulfide-linked member of the tetraspanin family that is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitor and is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma and is expressed in some autoimmune diseases where B cells participate in the pathology. Full-length human TSPAN33 shares 96% amino acid identity with mouse TSPAN33.
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