Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 545422
View all TSPAN33 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN33
Met1-Tyr283
Accession # NP_848657

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TSPAN33 PE‑conjugated Antibody

Detection of TSPAN33 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TSPAN33 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with overnight with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) for were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human TSPAN33 PE-conjugated Monoclonal Antibody (Catalog # FAB8405P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSPAN33 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with overnight with Recombinant Human IL‑4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSPAN33

TSPAN33 is a homodimeric disulfide-linked member of the tetraspanin family that is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitor and is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma and is expressed in some autoimmune diseases where B cells participate in the pathology. Full-length human TSPAN33 shares 96% amino acid identity with mouse TSPAN33.

Long Name

Tetraspanin 33

Alternate Names

PEN, Penumbra

Entrez Gene IDs

340348 (Human); 232670 (Mouse); 500065 (Rat)

Gene Symbol

TSPAN33

UniProt

NP_848657

Additional TSPAN33 Products

Product Documents for Human TSPAN33 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSPAN33 PE‑conjugated Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

Citations for Human TSPAN33 PE‑conjugated Antibody

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