Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 545422
View all TSPAN33 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN33
Met1-Tyr283
Accession # NP_848657

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TSPAN33 Antibody

Detection of TSPAN33 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TSPAN33 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated overnight with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) were stained with Mouse Anti-Human TSPAN33 Monoclonal Antibody (Catalog # MAB8405) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Applications for Human TSPAN33 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSPAN33

TSPAN33 is a member of the tetraspanin family and is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. TSPAN33 is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma. TSPAN33 is also expressed in some autoimmune diseases where B cells participate in the pathology.

Long Name

Tetraspanin 33

Alternate Names

PEN, Penumbra

Entrez Gene IDs

340348 (Human); 232670 (Mouse); 500065 (Rat)

Gene Symbol

TSPAN33

UniProt

NP_848657

Additional TSPAN33 Products

Product Documents for Human TSPAN33 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TSPAN33 Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

Citations for Human TSPAN33 Antibody

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