Human Tsukushi/TSK Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF3940AFP488

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all Tsukushi/TSK Primary Antibodies »

Product Specifications

Specificity

Detects human Tsukushi/TSK in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse TSK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Tsukushi/TSK Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Tsukushi/TSK

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (1, 2). All contain leucine-rich repeats (LRR) flanked by conserved cysteines, but the C-terminus of TSK resembles the structure of nyctalopin and chondroadherin rather than class I‑III SLRPs (1). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted (presumably glycosylated) protein that contains nine LRR (2). Proteoglycan modification of TSK has not been shown. SLRP-like activity or expression of TSK has been identified in several contexts. TSK mRNA is upregulated by estrogen treatment in breast cancer cell lines, and so is proposed to be involved in hormonally regulated extracellular matrix remodeling (2). TSK is upregulated along with bone markers in Vitamin K2-treated osteosarcoma cell lines, mediated by the pregnane X receptor, PXR (3). TSK was also shown to contribute to vitamin K2-mediated enhancement of collagen accumulation. Other SLRPs have been shown to form a protective coat around collagen fibrils, shielding them from proteolysis (4). In rats, expression of TSK in liver, intestines and islets of Langerhans resembles the pattern of Glut2 (5). TSK transcription is upregulated by both estrogen and insulin in hepatocytes. In Xenopus and chick embryos, TSK is expressed in the middle primitive streak, where it binds and antagonizes BMP-4 and promotes dorsalization and formation of the neural crest and the primitive organizer (6, 7). Xenopus TSK also binds the extracellular region of delta-1, modulating Notch activity (7). Human TSK shows 87%, 86%, 86%, 85%, 52% and 50% aa identity with canine, mouse, rat, bovine, chick, and Xenopus TSK, respectively, with the highest identity in the first four LRR.

References

  1. McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294.
  2. Charpentier, A.H. et al. (2000) Cancer Res. 60:5977.
  3. Ichikawa, T. et al. 2006, J. Biol. Chem. 281:16927.
  4. Geng, Y. et al. (2006) Matrix Biol. 25:484.
  5. Coffy, S. et al. (2005) Biochem. J. 385:165.
  6. Ohta, K. et al. (2004) Dev. Cell 7:347.
  7. Kuriyama, S. et al. (2006) Development 133:75.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Alternate Names

E2IG4, LRRC54, TSKU

Entrez Gene IDs

25987 (Human)

Gene Symbol

TSKU

UniProt

Q8WUA8

Additional Tsukushi/TSK Products

Product Documents for Human Tsukushi/TSK Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Tsukushi/TSK Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

BMP Family

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Protocols

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