Detection of ULBP‑3 in BaF3 Mouse Cell Line Transfected with Human ULBP-3 by Flow Cytometry.
BaF3 mouse pro-B cell line transfected with human ULBP-3 was stained with Mouse Anti-Human ULBP‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB1517A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).
Cosman, D. et al. (2001) Immunity 14:123.
Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
Sutherland, C. et al. (2002) J. Immunol. 168:671.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
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