Human ULBP-3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
  • Source
    Monoclonal Mouse IgG2A Clone # 166510
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human ULBP-3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ULBP‑3 in BaF3 Mouse Cell Line Transfected with Human ULBP-3 by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human ULBP-3 was stained with Mouse Anti-Human ULBP‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB1517A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-3

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).

  • References:
    1. Cosman, D. et al. (2001) Immunity 14:123.
    2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
    3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
    4. Steinle, A. et al. (2001) Immunogenetics 53:279.
    5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
    6. Pende, D. et al. (2002) Cancer Res. 62:6178.
    7. Radosavljevic, M. et al. (2002) Genomics 79:114.
    8. NKG2D and its Ligands (2002)
  • Long Name:
    UL16 Binding Protein-3
  • Entrez Gene IDs:
    79465 (Human)
  • Alternate Names:
    NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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