Human ULBP-3 PE-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
  • Source
    Monoclonal Mouse IgG2A Clone # 166510
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human ULBP-3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ULBP‑3 in Human monocyte-derived dendritic cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human ULBP‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of ULBP‑3 in BaF3 Mouse Cell Line Transfected with Human ULBP-3 by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human ULBP-3 was stained with Mouse Anti-Human ULBP‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-3

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).

  • References:
    1. Cosman, D. et al. (2001) Immunity 14:123.
    2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
    3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
    4. Steinle, A. et al. (2001) Immunogenetics 53:279.
    5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
    6. Pende, D. et al. (2002) Cancer Res. 62:6178.
    7. Radosavljevic, M. et al. (2002) Genomics 79:114.
    8. NKG2D and its Ligands (2002) http://www.RnDSystems.com/.
  • Long Name:
    UL16 Binding Protein-3
  • Entrez Gene IDs:
    79465 (Human)
  • Alternate Names:
    NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3; ULBP-3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
    Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
    Oncotarget, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
    Blood, 2016;127(23):2879-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
    Sci Rep, 2016;6(0):32470.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011;6(2):e16899.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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