Human ULBP-3 Antibody

Catalog # Availability Size / Price Qty
MAB1517-SP
MAB1517-500
MAB1517-100
Product Details
Citations (39)
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Human ULBP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
Source
Monoclonal Mouse IgG2A Clone # 166510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human ULBP‑3 transfected Baf/3 cells
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.3 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of biotinylated Recombinant Human ULBP-3 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-3

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) http://www.RnDSystems.com/.
Long Name
UL16 Binding Protein-3
Entrez Gene IDs
79465 (Human)
Alternate Names
NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3; ULBP-3

Product Datasheets

Citations for Human ULBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
    Authors: S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme
    Oncoimmunology, 2018;7(2):e1392426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells
    Authors: YS Lee, W Heo, J Nam, YH Jeung, J Bae
    J. Radiat. Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model
    Authors: NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz
    JCI Insight, 2017;2(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Purine nucleotide metabolism regulates expression of the human immune ligand MICA
    Authors: MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017;8(19):31959-31976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017;7(1):8661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
    Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
    Neoplasia, 2017;19(6):471-482.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
    Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
    Cell Death Dis, 2017;8(8):e2973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
    Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
    Eur J Immunol, 2015;45(8):2324-34.
    Species: Human
    Sample Types: Plasma
    Applications: Immunoprecipitation
  10. Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
    Authors: Baragano Raneros A, Martin-Palanco V, Fernandez A, Rodriguez R, Fraga M, Lopez-Larrea C, Suarez-Alvarez B
    Genes Immun, 2015;16(1):71-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
    Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
    BMC Cancer, 2015;15(0):351.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
    Authors: Kim, Ga Rim, Ha, Ga-Hee, Bae, Jae-Ho, Oh, Sae-Ock, Kim, Sun-Hee, Kang, Chi-Dug
    Oncol Lett, 2015;9(4):1641-1646.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
    Authors: Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prod'homme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P
    PLoS Pathog, 2014;10(5):e1004058.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Neutralization
  15. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
    Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A
    J Immunol, 2014;193(2):950-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014;32(1):290-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
    Authors: Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M
    Eur J Immunol, 2013;43(2):550-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
    Authors: Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K
    Cancer Res, 2013;73(8):2598-607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
    Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
    Cancer Res, 2012;72(21):5463-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012;86(16):8693-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012;92(7):967-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011;6(2):e16899.
  25. The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
    Authors: Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M
    J. Cell. Sci., 2011;124(0):321-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
    Authors: Delarosa O, Sanchez-Correa B, Morgado S, Ramirez C, del Rio B, Menta R, Lombardo E, Tarazona R, Casado JG
    Stem Cells Dev., 2011;21(8):1333-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2011;208(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
    Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
    Leukemia, 2010;24(6):1152-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  30. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
    Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
    Blood, 2010;115(25):5170-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
    Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
    Int. J. Cancer, 2009;124(7):1594-604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
    Authors: Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, Queirolo P, Vermi W, Facchetti F, Moretta A, Moretta L, Mingari MC, Vitale M
    Proc. Natl. Acad. Sci. U.S.A., 2009;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
    Authors: Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(37):15825-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  35. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  36. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008;45(15):3917-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
    Authors: Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
    Blood, 2007;110(4):1207-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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