Human VEGF Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2931V-100UG
Detection of VEGF in U937 Human Cell Line by Flow Cytometry
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Human VEGF Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF.
Source
Monoclonal Mouse IgG2A Clone # 23410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VEGF
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of VEGF in U937 Human Cell Line by Flow Cytometry View Larger

Detection of VEGF in U937 Human Cell Line by Flow Cytometry U937 human histiocytic lymphoma cell line either treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human VEGF Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # IC2931V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF) is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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