Human VEGF/PlGF Heterodimer DuoSet ELISA

• Assay Type
  Solid Phase Sandwich ELISA
• Format
  96-well strip plate
• Sample Volume Required
  100 µL
• Sufficient Materials
  For five 96-well plates*
• Specificity
  Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available
DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF / PlGF Heterodimer. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.


Product Features
• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
• Development protocols are provided to guide further assay optimization
• Assay can be customized to your specific needs
• Economical alternative to complete kits
Kit Content
• Capture Antibody
• Detection Antibody
• Recombinant Standard
• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent


*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Product Datasheets
Product Datasheet


COA
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SDS
Preparation and Storage
• Stability & Storage
  Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGF/PlGF Heterodimer
The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant PlGF/VEGF heterodimers are much less potent than VEGF homodimers as mitogens for endothelial cells.
• Long Name:
  Vascular Endothelial Growth Factor/Placenta Growth Factor Heterodimer
• Alternate Names:
  VEGF/PlGF Heterodimer

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