Human VEGF Quantikine QuicKit ELISA Summary
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Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human VEGF spiked to three levels in samples throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||99||94-104|
|EDTA Plasma (n=2)||83||79-88|
|Heparin Plasma (n=2)||77||70-81|
|Human Milk (n=2)||102||98-105|
Human VEGF Standard Curve
Human VEGF QuicKit ELISA Spiked Linearity Competitor Comparison Samples containing VEGF were diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-107% compared to 100%-145% for the top competitor.
Preparation and Storage
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3).
Citation for Human VEGF Quantikine QuicKit ELISA
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1 Citation: Showing 1 - 1
Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF
Authors: J Han, C Fang, P Ouyang, Y Qing, Y Yang, H Li, Z Wang, J Du
Sample Types: Serum
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