Human VEGFR1/Flt-1 Alexa Fluor® 488-conjugated Antibody

Name: Human VEGFR1/Flt-1 Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB321G-100UG
Rating: 5 (1 reviews)

FAB321G 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB321G-100UG

Request a Quote Bulk Orders

Human VEGFR1/Flt-1 Alexa Fluor® 488-conjugated Antibody in Data

1 Image

Product Details

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR1/Flt-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.

Source

Monoclonal Mouse IgG₁ Clone # 49560

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
Ser27-His687
Accession # NP_001153392

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

View Larger

Detection of VEGF R1/Flt‑1 in Human PBMC Monocytes by Flow Cytometry. Human PBMC Monocytes were stained with Mouse Anti-Human VEGF R1/Flt-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB321G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1