Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Cell Culture, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 49560
View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392

Specificity

Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VEGFR1/Flt-1 Antibody

Detection of VEGFR1/Flt-1 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of VEGFR1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human VEGFR1/Flt-1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB321 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

UniProt

NP_001153392

Additional VEGFR1/Flt-1 Products

Product Documents for Human VEGFR1/Flt-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human VEGFR1/Flt-1 Antibody

For research use only

Citations for Human VEGFR1/Flt-1 Antibody

Customer Reviews for Human VEGFR1/Flt-1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human VEGFR1/Flt-1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: retinal cells
    Species: Human
    Verified Customer | Posted 07/13/2024
    Human VEGFR1/Flt-1 Antibody MAB321
  • Human VEGFR1/Flt-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: endothelial cells
    Species: Human
    Verified Customer | Posted 09/10/2021
    Human VEGFR1/Flt-1 Antibody MAB321

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