Natural and recombinant human Lymphotactin. This assay also recognizes human XCL2.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Human XCL1/Lymphotactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lymphotactin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Lymphotactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lymphotactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lymphotactin.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates
The recovery of human Lymphotactin spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples spiked with high concentrations of human Lymphotactin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Lymphotactin (Lptn), also known as SCM-1a and ATAC, belongs to the C or gamma subfamily of chemokines and has been designated XCL1. Human Lymphotactin encodes a 114 amino acid (aa) precursor with a 21 aa predicted signal peptide. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
Chemokine [C Motif] Ligand 1
Entrez Gene IDs
6375 (Human); 16963 (Mouse);
ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin);