Human XCL1/Lymphotactin Quantikine ELISA Kit

    
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.023 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Lymphotactin. This assay also recognizes human XCL2.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC117 , Quantikine Immunoassay Control Set 816 Human Lymphotactin - Please Inquire
Product Summary
The Quantikine Human XCL1/Lymphotactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lymphotactin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Lymphotactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lymphotactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lymphotactin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.92 2.83 5.57 1.01 3.08 6.29
Standard Deviation 0.024 0.078 0.116 0.095 0.278 0.494
CV% 2.6 2.8 2.1 9.4 9 7.9

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.12 3.27 6.66 1.14 3.32 6.69
Standard Deviation 0.031 0.085 0.184 0.084 0.199 0.374
CV% 2.8 2.6 2.8 7.4 6 5.6

Recovery

The recovery of human Lymphotactin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 109 99-116
EDTA Plasma (n=4) 95 86-100
Heparin Plasma (n=4) 93 87-101
Serum (n=4) 98 89-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Lymphotactin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 XCL1/Lymphotactin [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: XCL1/Lymphotactin
Lymphotactin (Lptn), also known as SCM-1a and ATAC, belongs to the C or gamma subfamily of chemokines and has been designated XCL1. Human Lymphotactin encodes a 114 amino acid (aa) precursor with a 21 aa predicted signal peptide. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
  • Long Name:
    Chemokine [C Motif] Ligand 1
  • Entrez Gene IDs:
    6375 (Human); 16963 (Mouse)
  • Alternate Names:
    ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 816 Human Lymphotactin

Ctrl QC117
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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