ICAM-1/CD54 Antibody (020) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90586

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 020 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ICAM-1/CD54 (Accession#: NP_034623.1; Met 1-Asn 485).

Specificity

No cross-reactivity in ELISA with: Human ICAM1Human CD31Human ICAM3Human ICAM2Mouse VCAM1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ICAM-1/CD54 Antibody (020) - Azide and BSA Free

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse brain with rabbit monoclonal antibody (1:200). The image showing positive staining of choroid plexus.

Applications for ICAM-1/CD54 Antibody (020) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:100-1:500
Application Notes
This antibody will detect Human ICAM-1/CD54 in ELISA pair set (Catalog: # NBP2-79586).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (020) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ICAM-1/CD54 Antibody (020) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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