ID2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-88630
![Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP1-88630]](https://resources.rndsystems.com/images/products/ID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88630-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP1-88630]")
Key Product Details
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Scientific Data Images for ID2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP1-88630]
Immunocytochemistry/Immunofluorescence: ID2 Antibody [NBP1-88630] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.![ID2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ID2 Antibody - BSA Free [NBP1-88630]](https://resources.rndsystems.com/images/products/nbp1-88630_rabbit-polyclonal-id2-antibody-2562025911651.jpg "Chromatin Immunoprecipitation-exo-Seq: ID2 Antibody - BSA Free [NBP1-88630]")
Chromatin Immunoprecipitation-exo-Seq: ID2 Antibody - BSA Free [NBP1-88630]
ChIP-Exo-Seq composite graph for Anti-ID2 (NBP1-88630) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for ID2 Antibody - BSA Free
Chromatin Immunoprecipitation-exo-Seq
Immunocytochemistry/ Immunofluorescence
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Background: ID2
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Additional ID2 Products
Product Documents for ID2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for ID2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ID2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ID2 Antibody - BSA Free
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Q: Why there are 2 dominantly expressed bands in the Western Blot? Are there 2 different constructs used for expression in the HEK cells? Has the antibody been tested with endogenous ID2?
A: The ID2 overexpression lysate used for the western blot image of NBP1-88630 was NBL1-11814, which is supplied to us by a collaborator lab. As far as we know, there is only one construct expressing the ID2 protein. Since there are no bands in the negative control, we think that both bands detected in the lysate overexpressing the ID2 protein represent the target. I am sorry, but we have not investigated the identity of the two bands. Perhaps the lower band is a partially degraded version. We have received reports that NBP1-88630 has been successfully used in western blot with endogenous cell samples, although unfortunately we do not have the actual data. Nevertheless, the ICC/IF and IHC-P images for this antibody demonstrate that it can detect the endogenous protein.
Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
