ID4 Antibody (3F11) - Azide and BSA Free

Novus Biologicals | Catalog # H00003400-M13

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F11

Format

Azide and BSA Free
View all ID4 Primary Antibodies »

Product Specifications

Immunogen

ID4 (NP_001537.1, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARCKAAEAAADEPALCLQCDMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQLALETHPALLRQPPPPAPPHHPAGTCPAAPPRTPLTALNTDPAGAVNKQGDSILCR

Specificity

This product is specific for Human ID4 monoclonal antibody (M13), clone 3F11 [Gene ID: 3400].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ID4 Antibody (3F11) - Azide and BSA Free

Western Blot: ID4 Antibody (3F11) [H00003400-M13]

Western Blot: ID4 Antibody (3F11) [H00003400-M13]

Western Blot: ID4 Antibody (3F11) [H00003400-M13] - Analysis of ID4 expression in transfected 293T cell line by ID4 monoclonal antibody (M13), clone 3F11. Lane 1: ID4 transfected lysate (Predicted MW: 16.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: ID4 Antibody (3F11) [H00003400-M13] - Detection limit for recombinant GST tagged ID4 is 1 ng/ml as a capture antibody.

Applications for ID4 Antibody (3F11) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ID4

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Long Name

Inhibitor of DNA Binding 4

Alternate Names

Idb4

Gene Symbol

ID4

UniProt

NP_001537.1 (Human)

Additional ID4 Products

Product Documents for ID4 Antibody (3F11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ID4 Antibody (3F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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