IDO2 Antibody (OTI1A4)

Novus Biologicals | Catalog # NBP2-02730

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4
View all IDO2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDO2 Antibody (OTI1A4)

Western Blot: IDO2 Antibody (1A4) [NBP2-02730] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2.
Flow Cytometry: IDO2 Antibody (OTI1A4) [NBP2-02730]

Flow Cytometry: IDO2 Antibody (OTI1A4) [NBP2-02730]

Flow Cytometry: IDO2 Antibody (1A4) [NBP2-02730] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IDO2 antibody, and then analyzed by flow cytometry.
Flow Cytometry: IDO2 Antibody (OTI1A4) [NBP2-02730]

Flow Cytometry: IDO2 Antibody (OTI1A4) [NBP2-02730]

Flow Cytometry: IDO2 Antibody (1A4) [NBP2-02730] - Analysis of Jurkat cells, using anti-IDO2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for IDO2 Antibody (OTI1A4)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDO2

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM].

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Entrez Gene IDs

169355 (Human)

Gene Symbol

IDO2

Additional IDO2 Products

Product Documents for IDO2 Antibody (OTI1A4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IDO2 Antibody (OTI1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for IDO2 Antibody (OTI1A4)

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  • Q: I have a question regarding the use of the IDO2 antibody (NBP2-02730) for flow. Do you have any protocols or special recommendations for using this antibody for flow, since IDO2 is an intracellular enzyme?

    A:

    Although our general protocol posted on our website is not very specific, it could be referenced as the first step. Here is another protocol from R&D Systems (our sister company) addressing the steps of intracellular staining in flow

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