IDO2 Antibody (OTI1A4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72453

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Format

Azide and BSA Free
View all IDO2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDO2 Antibody (OTI1A4) - Azide and BSA Free

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IDO2 antibody, and then analyzed by flow cytometry.
Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2.
Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453]

Flow Cytometry: IDO2 Antibody (OTI1A4) - Azide and BSA Free [NBP2-72453] - Analysis of Jurkat cells, using anti-IDO2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for IDO2 Antibody (OTI1A4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IDO2

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 (PubMed 17499941)).(supplied by OMIM)

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Gene Symbol

IDO2

Additional IDO2 Products

Product Documents for IDO2 Antibody (OTI1A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IDO2 Antibody (OTI1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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