IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90685

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Sandwich ELISA Capture, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 062 expressed in HEK293

Format

Azide and BSA Free
View all IFN-gamma R1/CD119 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFN-gamma R1/CD119 (Uniprot#: P15261; Met1-Asp253).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685]

Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685]

Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] -

Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] -staining of mouse IFN-gamma R1/CD119 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90685 (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Applications for IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Sandwich ELISA Capture

1:250-1:2000
Application Notes
This antibody will detect Human IFN-gamma R1/CD119 in ELISA pair set (Catalog: # NBP2-79602).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-gamma R1/CD119

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Gene Symbol

IFNGR1

Additional IFN-gamma R1/CD119 Products

Product Documents for IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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