IgA Antibody (RM220) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25955
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM220 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Mouse IgA
Specificity
This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgA Antibody (RM220) - Azide and BSA Free
ELISA: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] -
ELISA: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] - ELISA of Mouse immunoglobulins shows RM220 reacts to Mouse IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.Western Blot: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] -
Western Blot: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] - Western blot of nonreduced(-) and reduced(+) Mouse IgA, using Clone RM220. This antibody reacts to nonreduced IgA.ELISA: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] -
ELISA: IgA Antibody (RM220) - Azide and BSA Free [NBP3-25955] - A titer ELISA of Mouse IgA. The plate was coated with different amount of Mouse IgA. A serial dilution of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.Applications for IgA Antibody (RM220) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.005 ug/ml - 0.2 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgA
Long Name
Immunoglobulin A
Alternate Names
Immunoglobulin A
Gene Symbol
IGHA1
Additional IgA Products
Product Documents for IgA Antibody (RM220) - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for IgA Antibody (RM220) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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