Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IGBP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 339) of human Protein Phosphatase 2A, regulatory subunit alpha-4 (Immunoglobulin binding protein 1) using the numbering given in SwissProt entry P78318 (GeneID 3476).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGBP1 Antibody - BSA Free

Immunoprecipitation: IGBP1 Antibody [NB100-487]

Immunoprecipitation: IGBP1 Antibody [NB100-487]

Immunoprecipitation: IGBP1 Antibody [NB100-487] - Detection of Alpha-4 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from Ramos (R), K562 (K) and mouse NIH3T3 (M) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP reaction loaded/lane) from Ramos cells. Antibodies: NB 100-487 used at 0.4 mcg/ml (A) and 1 mcg/ml (B) for WB at 3 mcg/mg lysate for IP. Alpha-4 was also immunoprecipitated by NB 100-486, but not by another Alpha-4 antibody, which are both affinity purified antibodies to upstream epitopes on Alpha-4. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).

Applications for IGBP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000. Immunoprecipitation: 2 to 5 ug/mg lysate. Histo/Cytochemistry: not tested. In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IGBP1

The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways.

Alternate Names

ALPHA-4, B cell signal transduction molecule alpha 4, bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ), B-cell signal transduction molecule alpha 4, CD79a-binding protein 1, IBP1, immunoglobulin (CD79A) binding protein 1, immunoglobulin-binding protein 1, Protein alpha-4, protein phosphatase 2A, regulatory subunit alpha-4, Renal carcinoma antigen NY-REN-16

Entrez Gene IDs

3476 (Human); 18518 (Mouse)

Gene Symbol

IGBP1

UniProt

P78318 (Human)

Additional IGBP1 Products

Product Documents for IGBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IGBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IGBP1 Antibody - BSA Free

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