IgD Antibody (RM123)

Novus Biologicals | Catalog # NBP2-62026

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM123 expressed in HEK293
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Product Specifications

Immunogen

Human IgD

Specificity

This antibody reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgD Antibody (RM123)

Immunohistochemistry: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62026.
IgD Antibody (RM123)

ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] -

ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] - ELISA of human immunoglobulins shows RM123 reacts to IgD from human plasma and IgD kappa from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgD Antibody (RM123)

ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] -

ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] - A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgD Antibody (RM123)

Sandwich ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] -

Sandwich ELISA: IgD Antibody (RM123) - Azide and BSA Free [NBP2-62026] - Sandwich ELISA using RM123 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

Applications for IgD Antibody (RM123)

Application
Recommended Usage

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.01 - 0.1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgD

Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Long Name

Immunoglobulin D

Alternate Names

Igh-5, Ighd, Immunoglobulin Delta

Gene Symbol

IGHD

Additional IgD Products

Product Documents for IgD Antibody (RM123)

Certificate of Analysis

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Product Specific Notices for IgD Antibody (RM123)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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