IGFBP-3 Antibody

Novus Biologicals | Catalog # NBP1-00203

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IGFBP-3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RYKVDYESQSTDTQN corresponding to internal region according to NP_000589.2.

Specificity

This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IGFBP-3 Antibody

Western Blot: IGFBP-3 Antibody [NBP1-00203]

Western Blot: IGFBP-3 Antibody [NBP1-00203]

Western Blot: IGFBP-3 Antibody [NBP1-00203] - (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IGFBP-3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.02 - 0.06 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human breast cancer lysates (calculated MW of 31.7 kDa band according to NP_001013416.1 and 32.2 kDa band according to NP_000589.2). An additional band of 50 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGFBP-3

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Entrez Gene IDs

3486 (Human); 16009 (Mouse); 24484 (Rat)

Gene Symbol

IGFBP3

UniProt

NP_001013416.1 (Human)

Additional IGFBP-3 Products

Product Documents for IGFBP-3 Antibody

Certificate of Analysis

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Product Specific Notices for IGFBP-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IGFBP-3 Antibody

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